DESENVOLVIMENTO DA TÉCNICA DE SEMI-NESTED-RT-PCR PARA A DETECÇÃO DO VIRUS DA DOENÇA DE NEWCASTLE EM POMBOS CAPTURADOS EM JABOTICABAL-SP, BRASIL / DEVELOPMENT OF SEMI-NESTED-RT-PCR TECHNIQUE FOR THE DETECTION OF NEWCASTLE DISEASE VIRUS IN PIGEONS ...
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چکیده
منابع مشابه
Differentiation of Virulent and Non-Virulent Newcastle Disease Virus Isolates Using RT-PCR
Newcastle disease is one of the main concerns of poultry farmers. Detection of virulent strains of Newcastle disease virus (NDV) has a great impact on control measures against the disease. In this study RT-PCR was optimized in high sensitivity in order to differentiate the virulent from non-virulent NDV isolates directly in tissue homogenates. The vaccinal NDV strain and known field isolates we...
متن کاملRapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR.
A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted N...
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The use of internet services has become a part of the people’s daily routine and should be sharply defined and with no technological restrains on its access and use. Elderly users now play an important role in the ever-growing online electronic communication devices not only through personal computers but also on tablets. The purpose of this research was evaluate how the elderly users managed G...
متن کاملDetection of Newcastle Disease Virus in Infected Chicken Embryos and Chicken Tissues by Rt-pcr
RT-PCR for the detection of Newcastle disease virus (NDV) in allantoic fluids of SPF embryonated eggs as well as in tissues of SPF chickens infected experimentally is described. The method proved to be specific as all tested NDVs were detected and no cross reaction with other RNA viruses was observed. Sensitivity of the method was established at 10ELD50/0.1 ml. To detect NDV in chicken tissues,...
متن کاملDetection and characterization of Newcastle disease virus in clinical samples using real time RT-PCR
Aim: Newcastle disease is still one of the major threats for poultry industry allover the world. Therefore, attempt was made in this study to use the SYBR Green I real-time PCR with melting curves analysis as for detection and differentiation of NDV strains in suspected infected birds. Materials and Methods: Two sets of primers were used to amplify matrix and fusion genes in samples collected f...
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ژورنال
عنوان ژورنال: Ars Veterinaria
سال: 2013
ISSN: 2175-0106,0102-6380
DOI: 10.15361/2175-0106.2013v29n4p11